Chromatography

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Definition:- Chromatography is the technique of separation of closely related compounds by a continuous redistribution of the mobile(liquid or gas) and the stationary phase(solid/liquid matrix) which interact through various physio chemical principles such as adsorption, partition, ion-exchange, molecular sieving, etc.

  • Types of Chromatography:-
    • Partition-
      • Paper-
        • 1D ascending
        • 1D descending
        • 2D
      • TLC
      • Gas-Liquid
    • Adsorption-
      • Column
      • TLC
    • Ion-exchange
    • Gel filtration
    • Affinity
    • HPLC- High Performance Liquid Chromatography
  • Rf Value:-
    • Ratio of fronts
    • Unique for different substances
  • Advantages:-
    • Simple, fast and economic method
    • Very small quantity of sample required
  • Applications:-
    • Qualitative assessment of proteins, carbohydrates, etc.
    • Identification of amino acidurias like PKU.
    • Detection of carbohydrates in metabolic disorders
    • Separation of isoenzymes